What is the principle of proximity alignment assay?

2.BODIPY-cholesterol

BODIPY-cholesterol is supplied as a crystalline solid. BODIPY-cholesterol Provided as a crystalline solid. A stock solution may be made by dissolving the BODIPY-cholesterol in the solvent of choice. Reserve solutions can be prepared by dissolving cholesterol in the selected solvent. BODIPY-cholesterol is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide(DMF), which should be purged with an inert gas. BODIPY-cholesterol Dissolve in organic solvents such as ethanol, DMSO, and dimethylformamide (DMF), which should be purified with inert gas. The solubility of BODIPY-cholesterol in ethanol is approximately 0.5 mg/ml and approximately 1 mg/ml in DMSO and DMF. BODIPY-cholesterol The solubility in ethanol is about 0.5 mg/ml, and the solubility in DMSO and DMF is about 1 mg/ml. BODIPY-cholesterol is sparingly soluble in aqueous buffers. BODIPY-cholesterol Soluble in water buffer solution. For maximum solubility in aqueous buffers, BODIPY-cholesterol should first be dissolved in DMSO and then diluted with the aqueous buffer of choice BODIPY-cholesterol has a solubility of approximately 0.5 mg/ml in a 1: 1 solution of DMSO: PBS(pH 7. 2) using this method. In order to achieve maximum solubility in water buffer, BODIPY cholesterol should first be dissolved in DMSO and then diluted with the selected water buffer. The solubility of BODIPY cholesterol in a DMSO: PBS (pH 7.2) 1:1 solution is approximately 0.5 mg/ml. We do not recommend telling the aqueous solution for more than one day. We do not recommend storing aqueous solutions for more than one day. BODIPY-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent BODIPY group at carbon 24.1.2 BODIPY-cholesterol It is a cholesterol analogue with biological activity and cell permeability, labeled with a fluorescent BODIPY group at carbon 24. It co-localizes with dehydroergosterol, a marker of cholesterol, in HeLa cells and is trafficked from the plasma membrane to the endocytic recycling compartment in BHK cells. 2 It co localizes with the cholesterol marker dehydroergosterol in HeLa cells and is transported from the plasma membrane of BHK cells to the endocytic circulation chamber. 2 BODIPY-cholesterol displays excitation/emission maxima of 480/508 nm, respectively, and has been used to monitor sterol uptake and inter-organelle sterol flux in cells BODIPY-cholesterol The maximum excitation/emission wavelengths displayed were 480/508 nm, respectively, and were used to monitor intracellular sterol uptake and inter organelle sterol flux. BODIPY-cholesterol (Glpbio, America) solutions were prepared in DMSO at a concentration of 5 BODIPY cholesterol (Glpbio, USA) was prepared as a 5 mM storage solution using DMSO. VSMCs were cultured in 6-well plates and labeled with 2.5 mM BODIPY cholesterol in culture medium for 2 hours at 37 ° C followed by washing with MEM-HEPES (Gibco, America). VSMC cells were cultured in 6-well plates at 37 ° C, labeled with 2.5 mM BODIPY cholesterol for 2 hours, and then washed with MEM-HEPES (Gibco, USA). After that, the efflux medium was removed and filtered through a 0.45  μm filter. The BODIPY fluorescence intensity was examined by using a fluorescence photometer (excitation 490 After removing the culture medium, filter it through a 0.45 μ m filter. Measure th

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